Detalji
UV wavelength selection
The UV tables offer the choice between three different UV wavelengths. The most commonly used wavelength for sample documentation is the midrange UV of 312 nm. It provides a sensitive signal detection with many fluorophores as ethidium bromide, SYBR® Gold, GelStar® or SYPRO® Orange and minimizes DNA photonicking compared to shorter wavelengths. The short wave 254 nm is preferred to achieve even higher sensitivity, e. g. when using SYBR® Green I dye. The long wave 365 nm is useful for excitation of e. g. Green Fluorescent Protein (GFP) and especially for preparative purposes.